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人抗乙型肝炎病毒核心IgM抗体酶免试剂盒,(HBcAb-IgM)ELISA检测试剂盒
产品时间:2016-10-02
人抗乙型肝炎病毒核心IgM抗体酶免试剂盒,(HBcAb-IgM)ELISA检测试剂盒,行业*,国内外多家ELISA试剂盒代理供应,价格相对平价,公司提供免费代测及ELISA试剂盒包邮服务。

本产品仅用于科研,不用于临床

人抗乙型肝炎病毒核心IgM抗体酶免试剂盒,(HBcAb-IgM)ELISA检测试剂盒 ,英文名:HBcAb-IgM ELISA Kit ,英文缩写:HBcAb-IgM ,常见试剂盒规格:96T/48T,产品别名:人抗乙型肝炎病毒核心IgM抗体ELISA检测试剂盒,HBcAb-IgM 检测试剂盒, HBcAb-IgM 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人抗乙型肝炎病毒核心IgM抗体酶免试剂盒,(HBcAb-IgM)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重 

 

周期素依赖性激酶4(CDK4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cyclin Dependent Kinase 4 (CDK4)48T/96TY09135Homo sapiens (Human)
杀菌性/通透性增加蛋白(BPI)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Bactericidal/Permeability Increasing Protein (BPI)48T/96TY09136Homo sapiens (Human)
毒蕈碱型胆碱受体M5(CHRM5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cholinergic Receptor, Muscarinic 5 (CHRM5)48T/96TY09137Homo sapiens (Human)
纤溶酶原(Plg)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Plasminogen (Plg)48T/96TY09138Homo sapiens (Human)
纤维蛋白肽A(FPA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibrinopeptide A (FPA)48T/96TY09139Homo sapiens (Human)
酰化刺激蛋白(ASP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Acylation Stimulating Protein (ASP)48T/96TY09140Homo sapiens (Human)
角蛋白19(KRT19)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Keratin 19 (KRT19)48T/96TY09141Homo sapiens (Human)
角蛋白20(KRT20)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Keratin 20 (KRT20)48T/96TY09142Homo sapiens (Human)
Kruppel样因子4(KLF4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kruppel Like Factor 4, Gut (KLF4)48T/96TY09143Homo sapiens (Human)
ATP结合盒转运蛋白A1(ABCA1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for ATP Binding Cassette Transporter A1 (ABCA1)48T/96TY09144Homo sapiens (Human)
利钾尿肽(KP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kaliuretic Peptide (KP)48T/96TY09145Homo sapiens (Human)
心型脂肪酸结合蛋白(FABP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fatty Acid Binding Protein 3, Muscle And Heart (FABP3)48T/96TY09146Homo sapiens (Human)
TGFβ诱导早期应答基因1(TIEG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for TGF Beta Inducible Early Response Gene 1 (TIEG1)48T/96TY09147Homo sapiens (Human)
胸腺表达趋化因子(TECK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Thymus Expressed Chemokine (TECK)48T/96TY09148Homo sapiens (Human)
雄激素受体(AR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Androgen Receptor (AR)48T/96TY09149Homo sapiens (Human)
TGFβ诱导早期应答基因1(TIEG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for TGF Beta Inducible Early Response Gene 1 (TIEG1)48T/96TY09150Mus musculus (Mouse)
血管抑素(ANG)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiostatin (ANG)48T/96TY09151Homo sapiens (Human)
延伸蛋白A(ELOA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Elongin A (ELOA)48T/96TY09152Homo sapiens (Human)
基质金属蛋白酶26(MMP26)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 26 (MMP26)48T/96TY09153Homo sapiens (Human)
26S-蛋白酶体(26S-PSM)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 26S-Proteasome (26S-PSM)48T/96TY09154Homo sapiens (Human)
谷氨酸脱羧酶2(GAD2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glutamate Decarboxylase 2, Acid (GAD2)48T/96TY09155Homo sapiens (Human)
Smad同源物7(Smad7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mothers Against Decapentaplegic Homolog 7 (Smad7)48T/96TY09156Mus musculus (Mouse)
接触蛋白3(CNTN3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Contactin 3 (CNTN3)48T/96TY09157Homo sapiens (Human)
胰岛素自身抗体(IAA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Insulin Autoantibody (IAA)48T/96TY09158Homo sapiens (Human)
胰多肽(PP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pancreatic Polypeptide (PP)48T/96TY09159Homo sapiens (Human)
胰高血糖素(GC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glucagon (GC)48T/96TY09160Homo sapiens (Human)
激肽原1(KNG1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kininogen 1 (KNG1)48T/96TY09161Homo sapiens (Human)
胱天蛋白酶13(CASP13)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Caspase 13 (CASP13)48T/96TY09162Bos taurus; Bovine (Cattle)
孕酮受体(PGR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Progesterone Receptor (PGR)48T/96TY09163Homo sapiens (Human)
早老素2(PS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Presenilin 2 (PS2)48T/96TY09164Homo sapiens (Human)
膜辅蛋白(MCP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Membrane Cofactor Protein (MCP)48T/96TY09165Homo sapiens (Human)
激素敏感性脂肪酶(LIPE)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lipase, Hormone Sensitive (LIPE)48T/96TY09166Homo sapiens (Human)
脑型脂肪酸结合蛋白(FABP7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fatty Acid Binding Protein 7, Brain (FABP7)48T/96TY09167Homo sapiens (Human)
组织多肽特异性抗原(TPS)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tissue Polypeptide Specific Antigen (TPS)48T/96TY09168Homo sapiens (Human)
Smad同源物7(Smad7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mothers Against Decapentaplegic Homolog 7 (Smad7)48T/96TY09169Rattus norvegicus (Rat)
Smad同源物7(Smad7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mothers Against Decapentaplegic Homolog 7 (Smad7)48T/96TY09170Homo sapiens (Human)
Smad同源物1(Smad1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mothers Against Decapentaplegic Homolog 1 (Smad1)48T/96TY09171Mus musculus (Mouse)
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含血管性血友病因子A域蛋白3A(vWA3A)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Von Willebrand Factor A Domain Containing Protein 3A (vWA3A)48T/96TY09174Homo sapiens (Human)
Ⅰ型胶原交联氨基端肽(NTXⅠ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cross Linked N-opeptide Of Type I Collagen (NTXI)48T/96TY09175Homo sapiens (Human)
分泌型免疫球蛋白A(sIgA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secretory Immunoglobulin A (sIgA)48T/96TY09176Homo sapiens (Human)
果糖二磷酸醛缩酶A(ALDOA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aldolase A, Fructose Bisphosphate (ALDOA)48T/96TY09177Homo sapiens (Human)
27kDa热休克蛋白(HSPB1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 27kDa Protein 1 (HSPB1)48T/96TY09178Homo sapiens (Human)
27kDa热休克蛋白(HSPB1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 27kDa Protein 1 (HSPB1)48T/96TY09179Rattus norvegicus (Rat)
27kDa热休克蛋白(HSPB1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Heat Shock 27kDa Protein 1 (HSPB1)48T/96TY09180Mus musculus (Mouse)
Ⅰ型胶原交联氨基端肽(NTXⅠ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cross Linked N-opeptide Of Type I Collagen (NTXI)48T/96TY09181Mus musculus (Mouse)
Ⅰ型胶原交联氨基端肽(NTXⅠ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cross Linked N-opeptide Of Type I Collagen (NTXI)48T/96TY09182Rattus norvegicus (Rat)
50kDa核孔蛋白(NUP50)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nucleoporin 50kDa (NUP50)48T/96T Y09183Homo sapiens (Human)
CCAAT增强子结合蛋白α(CEBPα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for CCAAT/Enhancer Binding Protein Alpha (CEBPa)48T/96TY09184Homo sapiens (Human)

 

各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。

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