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小鼠钩端螺旋体IgG酶免试剂盒,(Lep IgG)ELISA检测试剂盒 ,英文名:Lep IgG ELISA Kit ,英文缩写:Lep IgG ,常见试剂盒规格:96T,产品别名:小鼠钩端螺旋体IgGELISA检测试剂盒,Lep IgG 检测试剂盒, Lep IgG 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠钩端螺旋体IgG酶免试剂盒,(Lep IgG)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
溶质载体家族30成员4(SLC30A4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Solute Carrier Family 30 Member 4 (SLC30A4)48T/96TY16485Mus musculus (Mouse)
溶质载体家族30成员7(SLC30A7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Solute Carrier Family 30 Member 7 (SLC30A7)48T/96TY16486Mus musculus (Mouse)
鞘氨醇1磷酸酯裂解酶1(SGPL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sphingosine 1 Phosphate Lyase 1 (SGPL1)48T/96TY16487Mus musculus (Mouse)
11-β-羟基类固醇脱氢酶2(HSD11β2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2)48T/96TY16488Homo sapiens (Human)
L-型电压依赖钙离子通道α1S亚基(CACNα1S)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S)48T/96TY16489Mus musculus (Mouse)
L-型电压依赖钙离子通道α1C亚基(CACNα1C)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C)48T/96TY16490Mus musculus (Mouse)
L-型电压依赖性钙离子通道α1D亚基(CACNα1D)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D)48T/96TY16491Mus musculus (Mouse)
L-型电压依赖钙离子通道α1F亚基(CACNα1F)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calcium Channel, Voltage Dependent, L-Type, Alpha 1F Subunit (CACNa1F)48T/96TY16492Mus musculus (Mouse)
N-型电压依赖钙离子通道α1B亚基(CACNα1B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B)48T/96TY16493Mus musculus (Mouse)
R-型电压依赖钙离子通道α1E亚基(CACNα1E)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E)48T/96TY16494Mus musculus (Mouse)
T-型电压依赖钙离子通道α1H亚基(CACNα1H)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H)48T/96TY16495Mus musculus (Mouse)
端粒重复结合因子2(TERF2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for omeric Repeat Binding Factor 2 (TERF2)48T/96TY16496Homo sapiens (Human)
过氧化还原酶6(PRDX6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Peroxiredoxin 6 (PRDX6)48T/96TY16497Mus musculus (Mouse)
钠/钾离子转运ATP酶α1肽(ATP1α1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)48T/96TY16498Rattus norvegicus (Rat)
钠/钾离子转运ATP酶β1肽(ATP1β1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1)48T/96TY16499Rattus norvegicus (Rat)
溶质载体家族30成员3(SLC30A3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Solute Carrier Family 30, Member 3 (SLC30A3)48T/96TY16500Mus musculus (Mouse)
溶质载体家族30成员5(SLC30A5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Solute Carrier Family 30, Member 5 (SLC30A5)48T/96TY16501Mus musculus (Mouse)
溶质载体家族30成员6(SLC30A6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Solute Carrier Family 30, Member 6 (SLC30A6)48T/96TY16502Mus musculus (Mouse)
肝细胞核因子1β(HNF1β)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hepatocyte Nuclear Factor 1 Beta (HNF1b)48T/96TY16503Rattus norvegicus (Rat)
丙糖磷酸异构酶1(TPI1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Triosephosphate Isomerase 1 (TPI1)48T/96TY16504Mus musculus (Mouse)
墨蝶呤还原酶(SPR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sepiapterin Reductase (SPR)48T/96TY16505Mus musculus (Mouse)
亮氨酸丰富重复FLII相互作用蛋白1(LRRFIP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1)48T/96TY16506Homo sapiens (Human)
端粒保护1同源物(POT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protection Of omeres 1 Homolog (POT1)48T/96TY16507Homo sapiens (Human)
含亮氨酸丰富重复蛋白3C(LRRC3C)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C)48T/96TY16508Homo sapiens (Human)
FK506结合蛋白样蛋白(FKBPL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for FK506 Binding Protein Like Protein (FKBPL)48T/96TY16509Mus musculus (Mouse)
无翅型MMTV整合位点家族成员4(WNT4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 4 (WNT4)48T/96TY16510Homo sapiens (Human)
含RCC1和BTB域蛋白2(RCBTB2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for RCC1 And BTB Domain Containing Protein 2 (RCBTB2)48T/96TY16511Homo sapiens (Human)
类固醇5α还原酶2(SRD5α2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2)48T/96TY16512Homo sapiens (Human)
含SET域蛋白7(SETD7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for SET Domain Containing Protein 7 (SETD7)48T/96TY16513Homo sapiens (Human)
赫曼斯基普德拉克综合征蛋白6(HPS6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hermansky Pudlak Syndrome Protein 6 (HPS6)48T/96TY16514Homo sapiens (Human)
丝氨酸蛋白酶50(PRSS50)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protease, Serine 50 (PRSS50)48T/96TY16515Homo sapiens (Human)
含Toll白细胞介素1受体域衔接蛋白(TIRAP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP)48T/96TY16516Homo sapiens (Human)
肌球蛋白重链7B(MYH7B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B)48T/96TY16517Homo sapiens (Human)
无翅型MMTV整合位点家族成员9B(WNT9B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 9B (WNT9B)48T/96TY16518Homo sapiens (Human)
T-框蛋白21(TBX21)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for T-Box Protein 21 (TBX21)48T/96TY16519Homo sapiens (Human)
粘蛋白12(MUC12)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Mucin 12, Cell Surface Associated (MUC12)48T/96TY16520Homo sapiens (Human)
钠/钾离子转运ATP酶β4肽(ATP1β4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4)48T/96TY16521Rattus norvegicus (Rat)
棘皮动物微管关联蛋白样蛋白2(EML2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Echinoderm Microtubule Associated Protein Like Protein 2 (EML2)48T/96TY16522Homo sapiens (Human)
胎盘特异性蛋白9(PLAC9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Placenta Specific Protein 9 (PLAC9)48T/96TY16523Homo sapiens (Human)
短腭肺鼻腔上皮癌关联蛋白2(SPLUNC2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2)48T/96TY16524Homo sapiens (Human)
短腭肺鼻腔上皮癌关联蛋白3(SPLUNC3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 (SPLUNC3)48T/96TY16525Homo sapiens (Human)
抑制素βC(INHβC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Inhibin Beta C (INHbC)48T/96TY16526Mus musculus (Mouse)
铜伴侣超氧化物歧化酶(SOD4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Superoxide Dismutase, Copper Chaperone (SOD4)48T/96TY16527Homo sapiens (Human)
胱天蛋白酶3(CASP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Caspase 3 (CASP3)48T/96TY16528Bos taurus; Bovine (Cattle)
补体受体2(CR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Complement Receptor 2 (CR2)48T/96TY16529Rattus norvegicus (Rat)
细胞程序性死亡蛋白1(PDCD1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Programmed Cell Death Protein 1 (PDCD1)48T/96TY16530Homo sapiens (Human)
肌球蛋白重链2(MYH2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)48T/96TY16531Homo sapiens (Human)
B-细胞淋巴瘤因子2(Bcl2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2)48T/96TY16532Bos taurus; Bovine (Cattle)
甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Parathyroid Hormone Related Protein (PTHrP)48T/96TY16533Bos taurus; Bovine (Cattle)
整合素α2(ITGα2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Integrin Alpha 2 (ITGa2)48T/96TY16534Rattus norvegicus (Rat)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。